Enzyme families in the Repository
Constructs are organised by the sequence-based families of the CAZy classification, with glycan-related enzymes that CAZy does not classify grouped by function instead. 115 families hold 395 listings covering 383 distinct records — a gene assigned to two families appears under both. The count beside each family is the number of entries it holds.
Glycosyltransferases
Glycosyltransferases transfer a sugar from an activated donor onto an acceptor. CAZy groups them into sequence-based families, which is the organising axis used here.
GT123GT26GT32GT45GT63GT715GT89GT108GT112GT122GT132GT1410GT161GT171GT182GT211GT224GT231GT242GT253GT2720GT2920GT3125GT322GT331GT353GT392GT411GT433GT474GT493GT501GT544GT572GT581GT591GT612GT645GT651GT662GT681GT761GT903
| Family | Entries | Enzyme specificities |
|---|---|---|
| GT1 | 23 | UDP glucuronosyltransferase, GlcNAc transferase |
| GT2 | 6 | dolichyl-phosphate β-D-mannosyltransferase, dolichyl-phosphate β-glucosyltransferase, N-acetylglucosaminyltransferase, hyaluronan synthase |
| GT3 | 2 | Glycogen Synthase |
| GT4 | 5 | GDP-Man α-mannosyltransferase, phosphatidylinositol N-acetylglucosaminyltransferase |
| GT6 | 3 | α-1,3-galactosyltransferase, α-1,3 N-acetylgalactosaminyltransferase, globoside α-N-acetylgalactosaminyltransferase |
| GT7 | 15 | lactose synthase, β-N-acetylglucosaminyl-glycopeptide β-1,4-galactosyltransferase, N-acetyllactosamine synthase, β-1,4-N-acetylglucosaminyltransferase, xylosylprotein β-4-galactosyltransferase, Chondroitin synthase |
| GT8 | 9 | glucoside xylosyltransferase, glycogenin glucosyltransferase, matriglycan xylosyltransferase, xyloside xylosyltransferase |
| GT10 | 8 | galactoside α-1,3/1,4-L-fucosyltransferase |
| GT11 | 2 | GDP-L-Fuc: galactoside α-1,2-L-fucosyltransferase |
| GT12 | 2 | beta-1,4-N-acetyl-galactosaminyl transferase |
| GT13 | 2 | mannose beta1,2-N-acetylglucosaminyltransferase |
| GT14 | 10 | β-1,6-N-acetylglucosaminyltransferase, protein O-β-xylosyltransferase |
| GT16 | 1 | mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase |
| GT17 | 1 | mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase |
| GT18 | 2 | mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase |
| GT21 | 1 | UDP-glucose ceramide glucosyltransferase |
| GT22 | 4 | Dol-P-Man α-mannosyltransferase |
| GT23 | 1 | alpha (1,6) fucosyltransferase |
| GT24 | 2 | UDP-glucose glycoprotein glucosyltransferase |
| GT25 | 3 | procollagen galactosyltransferase |
| GT27 | 20 | polypeptide α-N-acetylgalactosaminyltransferase |
| GT29 | 20 | CMP-NeuAc sialyltransferases |
| GT31 | 25 | β-1,3-N-acetylgalactosaminyltransferase, β-1,3-galactosyltransferase, β-1,3-N-acetylglucosaminyltransferase, chondroitin β-1,4-N-acetylgalactosaminyltransferase |
| GT32 | 2 | alpha-1,4-N-acetylglucosaminyltransferase, alpha 1,4-galactosyltransferase |
| GT33 | 1 | beta-1,4-mannosyltransferase |
| GT35 | 3 | glycogen phosphorylase |
| GT39 | 2 | protein-O-mannosyltransferase |
| GT41 | 1 | O-linked N-acetylglucosamine (GlcNAc) UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase |
| GT43 | 3 | beta-1,3-glucuronyltransferase |
| GT47 | 4 | heparan β-1,4-glucuronyltransferase, heparan synthase |
| GT49 | 3 | β-1,4-glucuronyltransferase |
| GT50 | 1 | GPI alpha-1,4-mannosyltransferase |
| GT54 | 4 | mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase |
| GT57 | 2 | alpha-1,3-glucosyltransferase |
| GT58 | 1 | alpha-1,3-mannosyltransferase |
| GT59 | 1 | alpha-1,2-glucosyltransferase |
| GT61 | 2 | N-acetyl-galactosaminyl transferase |
| GT64 | 5 | heparan α-N-acetylglucosaminyltransferase |
| GT65 | 1 | protein O-fucosyltransferase |
| GT66 | 2 | STT3, subunit of the oligosaccharyltransferase complex |
| GT68 | 1 | protein O-fucosyltransferase |
| GT76 | 1 | GPI alpha-1,6-mannosyltransferase |
| GT90 | 3 | protein O-glucosyltransferase |
Glycoside hydrolases
Glycoside hydrolases cleave glycosidic bonds. Most of the mammalian enzymes in this collection are lysosomal or Golgi-resident.
GH15GH22GH135GH186GH203GH225GH232GH272GH292GH301GH317GH334GH354GH371GH385GH391GH477GH565GH591GH631GH651GH792GH841GH851GH891GH992GH1161GH1331
| Family | Entries | Enzyme specificities |
|---|---|---|
| GH1 | 5 | Lactase, beta-glucosidase |
| GH2 | 2 | beta-glucuronidase, beta-mannosidase |
| GH13 | 5 | amylase, glucan (1,4-alpha-), branching/debranching enzymes, solute carrier protein |
| GH18 | 6 | chitinase |
| GH20 | 3 | beta-hexosamindase |
| GH22 | 5 | lysozyme |
| GH23 | 2 | lysozyme-like |
| GH27 | 2 | alpha-galactosidase, alpha-N-acetylglucosamindase |
| GH29 | 2 | Alpha-L-fucosidase |
| GH30 | 1 | acid beta-glucosidase |
| GH31 | 7 | alpha-glucosdase |
| GH33 | 4 | sialidase |
| GH35 | 4 | beta-galactosidase |
| GH37 | 1 | trehalase |
| GH38 | 5 | alpha-mannosidase |
| GH39 | 1 | alpha-L-iduronidase |
| GH47 | 7 | alpha-mannosidase |
| GH56 | 5 | hyaluronoglucosaminidase |
| GH59 | 1 | galactosylceramidase |
| GH63 | 1 | glucosidase 1 |
| GH65 | 1 | acid trehalase |
| GH79 | 2 | heparanase |
| GH84 | 1 | protein N-acetylglucosamindase |
| GH85 | 1 | endo-beta-N-acetylglucosaminidase |
| GH89 | 1 | alpha-N-acetylglucosaminidase |
| GH99 | 2 | endo-alpha-mannosidase |
| GH116 | 1 | glucoceramidase |
| GH133 | 1 | glycogen debranching enzyme |
Other glycan-related enzymes
Glycan-related enzymes that CAZy does not classify: sulfotransferases, sulfatases, phosphotransferases, protein disulfide isomerases, ER lectins and chaperones, and the enzymes of dolichol-phosphate sugar synthesis.
Glucosidase 2 beta subunit1HSP70 (GRP-78)1Dol-P-Man synthase-associated subunits2Dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)1ER quality control lectins2GlcNAc-PO4 transferase subunits2Matriglycan linker region enzymes5N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase1Protein disulfide isomerase5Sulfatases4Sulfotransferases and epimerases35
Bacterial glycosyltransferases
Bacterial glycosyltransferase homologues cloned for expression in E. coli, selected as tractable surrogates for the mammalian enzymes.
| Family | Entries | Enzyme specificities |
|---|---|---|
| GT1 | 1 | UDP glucuronosyltransferase?? |
| GT2 | 3 | dolichyl-phosphate β-D-mannosyltransferase |
| GT3 | 2 | Glycogen Synthase |
| GT4 | 1 | UDP-GlcNAc:1L-myo-inositol-1-P-aalpha-N-acetylglucoaminyltransferase |
| GT7 | 1 | β-1,4-galactosyltransferase |
| GT10 | 1 | fucosyltransferase |
| GT11 | 1 | GDP-L-Fuc: galactoside α-1,2-L-fucosyltransferase |
| GT12 | 1 | beta-1,4-N-acetyl-galactosaminyl transferase |
| GT21 | 1 | beta-galactosyltransferase |
| GT25 | 2 | β-1,3-glucosyltransferase, β-1,2-glucosyltransferase, β-1,2-galactosyltransferase??? |
| GT27 | 1 | polypeptide α-N-acetylgalactosaminyltransferase ??? |
| GT35 | 1 | maltodextrin phosphorylase |
| GT39 | 1 | protein-O-mannosyltransferase |
| GT41 | 1 | O-linked N-acetylglucosamine (GlcNAc) UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase |
| GT66 | 1 | subunit of the oligosaccharyltransferase complex |
Bacterial glycoside hydrolases
Bacterial glycoside hydrolase homologues cloned for expression in E. coli.
| Family | Entries | Enzyme specificities |
|---|---|---|
| GH1 | 2 | beta-glucosidase |
| GH2 | 3 | beta-mannosidase |
| GH13 | 3 | oligo-1,6-alpha-glucosidase |
| GH18 | 1 | chitinase |
| GH27 | 1 | alpha-galactosidase |
| GH29 | 2 | Alpha-L-fucosidase |
| GH30 | 1 | beta-glucosidase |
| GH31 | 1 | alpha-glucosdase |
| GH33 | 1 | sialidase |
| GH35 | 3 | beta-galactosidase |
| GH37 | 1 | trehalase |
| GH38 | 1 | alpha-mannosidase |
| GH39 | 1 | alpha-L-iduronidase |
| GH47 | 1 | alpha-mannosidase |
| GH84 | 1 | beta-N-acetylglucosaminidase |
| GH85 | 1 | endo-beta-N-acetylglucosaminidase |
| GH89 | 3 | alpha-N-acetylglucosaminidase |
| GH99 | 1 | alpha-mannosidase |