Glyco-Enzyme Repository

Repository of Expression Constructs for Glycosylation Enzymes

Gateway® entry clones and mammalian, baculovirus and bacterial expression constructs for glycosyltransferases, glycoside hydrolases and glycan-modifying enzymes

Data notes and curation worklist

Migrating the site required reading every record mechanically, which exposed places where the original data is internally inconsistent or incomplete. Nothing here has been corrected — the migration preserves the source as it stands. This page lists what a revision pass should look at.

15Identifier conflicts
27Naming differences
36Missing files
4Duplicated symbols
114No CAZy family

Conflicting identifiers

The legacy site stated each gene's identity twice: in the family index that links to the record, and in the record's own header table. For these 15 values the two disagree in a way that is not merely a naming or formatting difference. One of the two is wrong in each case; the migration does not guess which, and both are shown on the affected record.

RecordFamily indexFieldValue in family indexValue in record
KLBGH1UniProt accessionQ86Z14Q49AQ7
AMY1A, AMY1B, AMY1C, AMY2A, AMY2BGH13Gene symbolAMY1A/B/C/AMY2A/BAMY1A, AMY1B, AMY1C, AMY2A, AMY2B
AMY1A, AMY1B, AMY1C, AMY2A, AMY2BGH13NCBI GeneID276/277/278/279/280NP_004029, NP_001008219, NP_001008220, NP_000690, NP_066188, NP_001008222
LYZL1, LYZL2GH22Gene symbolLYZL1LYZL1, LYZL2
LYZL1, LYZL2GH22NCBI GeneID84569/11918084569, 119180
GCNT2CGT14UniProt accessionQ8NFS9Q06430
UGCGL2GT24Gene symbolUGGT2UGCGL2
ALG8GT57Gene symbolALG6ALG8
ALG8GT57UniProt accessionQ9Y672Q9BVK2
ALG8GT57NCBI GeneID2992979053
KDELC2GT90Gene symbolKTELC2KDELC2
POGLUT1GT90UniProt accessionQ8NBL1C9JYJ3
POGLUT1GT90NCBI GeneID56983Gene Synthesis
POMKOtherMatriglycanLinkerUniProt accessionQ9H5K3Q8TE99
POMKOtherMatriglycanLinkerNCBI GeneID8419792370
The most consequential of these is the GT57 record. Its own header table reads ALG8 / Q9BVK2 / GeneID 79053, but the GT57 family index calls it ALG6 / Q9Y672 / GeneID 29929, and every construct file on the record is named ALG6-*. The file names and the family index agree with each other against the header table, so the header table is the likely error — but that is a curation decision, not a migration one, so both readings are presented.

Superseded gene symbols

Gene nomenclature has moved on since these records were written. Where a record carries an older symbol alongside the current one — recorded in the source as a parenthesised alternative — both are kept and both are searchable. These are naming differences rather than conflicts, but they mark records whose annotation is likely to be out of date.

Symbol on recordAlso recorded asUniProtAnnotation
AGLGDEP35573Glycogen debranching enzyme
B3GALNT1B3GALT3O75752UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
B3GLCTB3GALTLQ6Y288Beta-1,3-glucosyltransferase
CHST14D4ST1Q8NCH0Carbohydrate sulfotransferase 14: synthesis of GalNAc-4-SO4 in dermatan sulfate
CHST14D4ST1Q8NCH0Carbohydrate sulfotransferase 14: synthesis of GalNAc-4-SO4 in dermatan sulfate
CHST15GALNAC4S6STQ7LFX5Carbohydrate sulfotransferase 15: transfers to 6 position of GalNAc4-SO4 in chondroitin sulfate
COLGALT1GLT25D1Q8NBJ5Procollagen galactosyltransferase 1
COLGALT2GLT25D2Q8IYK4Procollagen galactosyltransferase 2
EOGTAER61Q5NDL2EGF domain-specific O-linked N-acetylglucosamine transferase
GALNT15GALNTL2Q8N3T1Polypeptide N-acetylgalactosaminyltransferase 15
GALNT16GALNTL1Q8N428Polypeptide N-acetylgalactosaminyltransferase 16
GALNT17GALNTL6Q49A17Polypeptide N-acetylgalactosaminyltransferase 17
GALNT18GALNTL4Q6P9A2Polypeptide N-acetylgalactosaminyltransferase 18
GALNT20GALNTL5Q7Z4T8Polypeptide N-acetylgalactosaminyltransferase 20 (Inactive polypeptide N-acetylgalactosaminyltransferase-like protein 5)
GXYLT1GLT8D3Q4G148Glucoside xylosyltransferase 1
GXYLT2GLT8D4A0PJZ3Glucoside xylosyltransferase 2
LARGE2GYLTL1BQ8N3Y3Glycosyltransferase-like protein LARGE2
MGAT4DGNT1IP-SA6NG13Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like protein MGAT4D
MOGSGCS1Q13724Mannosyl-oligosaccharide glucosidase
OGAMGEA5O60502Protein O-GlcNAcase and inactive Histone acetyltransferase
POGLUT1KTELC1, RUMIC9JYJ3Protein O-glucosyltransferase 1
POMGNT2GTDC2, AGO61, EOGTLQ8NAT1Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2
RTFDC1C20orf43Q9BY42Protein RTF2 homolog
UGGT1UGCGL1Q9NYU2UDP-glucose:glycoprotein glucosyltransferase 1
WBSCR17GALNT19, GALNTL3Q6IS24Polypeptide N-acetylgalactosaminyltransferase 19 (Williams-Beuren syndrome chromosome region 17 protein)
XXYLT1C3orf21Q8NBI6Xyloside xylosyltransferase 1

Symbols held by more than one record

4 legacy record pages share a gene-symbol value with another page. Each is kept as its own record, named after the source page it came from so the two can be told apart. They need reconciling: either they are two different genes, one of them mislabelled, or they are duplicate entries for one gene.

Symbol in sourceRecordBuilt fromUniProtGeneIDConstruct directories
ALG8openALG8.htmlQ9BVK279053ALG8
ALG8openALG6.htmlQ9BVK279053ALG6
CHST14 (D4ST1)openCHST14.htmlQ8NCH0113189Nterm tag
CHST14 (D4ST1)openD4ST1.htmlQ8NCH0113189Nterm tag
The two ALG8 records carry the same UniProt accession and GeneID but reference entirely different construct files — one set named ALG6-*, the other ALG8-*. ALG6 and ALG8 are distinct genes, so the likeliest reading is one correct ALG8 record plus an ALG6 record whose header table was filled in from the wrong gene. The CHST14 pair agrees on every identifier and looks instead like a genuine duplicate entry, recorded once under each of the gene's two names.

Records with no CAZy family

114 records carry no usable CAZy family label. Most are the glycan-modifying enzymes CAZy does not classify, which is expected and correct — they are grouped by function instead. A few, listed below with the raw value found in the source, have a malformed entry in that field.

RecordRaw value in the CAZy fieldEnzyme class
AMY1A, AMY1B, AMY1C, AMY2A, AMY2BBC144452Glycoside hydrolase
GYS13Glycosyltransferase

Referenced files that do not exist

36 file references across 13 records point at files the original server did not hold; requesting them returned a 404 there too, so there was nothing to migrate. They are shown on the records as unavailable rather than removed, since the reference is evidence the construct was made.

RecordFilesFile names
B3GALNT11B3GALT3-AcGT N-term MelHisStrep-DEST.gbk
COLGALT27GLT25D2-Nterm-TEVfusion-pDONR221.gbk; GLT25D2-C-term-TEVfusion-pDONR221.gbk; GLT25D2-pGEn2-DEST.gbk; GLT25D2-pGEc1-DEST.gbk; GLT25D2-pGEc2-DEST.gbk; GLT25D2-AcGH C-term HisStrep-DEST.gbk; GLT25D2-AcGT N-term MelHisStrep-DEST.gbk
EOGT1AER61-AcGT N-term MelHisStrep-DEST.gbk
GCNT2A1GCNT2-AcGT N-term MelHisStrep-DEST.gbk
GCNT2B1GCNT2-AcGT N-term MelHisStrep-DEST.gbk
GCNT2C1GCNT2-AcGT N-term MelHisStrep-DEST.gbk
GXYLT11GLT8D3-AcGT N-term MelHisStrep-DEST.gbk
GXYLT21GLT8D3-AcGT N-term MelHisStrep-DEST.gbk
LARGE11LARGE-AcGT N-term MelHisStrep-DEST.gbk
OGA5MGEA5-Nterm-TEVfusion-pDONR221.gbk; MGEA5-pGEn1-DEST.gbk; MGEA5-pGEn2-DEST.gbk; MGEA5-pGEn3-DEST.gbk; MGEA5-AcGT N-term MelHisStrep-DEST.gbk
POGLUT12KTELC1-AcGH C-term HisStrep-DEST.gbk; KTELC1-AcGT N-term MelHisStrep-DEST.gbk
RTFDC19C20orf43-Nterm-TEVfusion-pDONR221.gbk; C20orf43-C-term-TEVfusion-pDONR221.gbk; C20orf43-pGEn1-DEST.gbk; C20orf43-pGEn2-DEST.gbk; C20orf43-pGEn3-DEST.gbk; C20orf43-pGEc1-DEST.gbk; C20orf43-pGEc2-DEST.gbk; C20orf43-AcGH C-term HisStrep-DEST.gbk; C20orf43-AcGT N-term MelHisStrep-DEST.gbk
XXYLT15C3orf21-Nterm-TEVfusion-pDONR221.gbk; C3orf21-pGEn1-DEST.gbk; C3orf21-pGEn2-DEST.gbk; C3orf21-pGEn3-DEST.gbk; C3orf21-AcGT N-term MelHisStrep-DEST.gbk

Records with no orderable clone

1 records describe constructs for which no DNASU clone ID and no Jarvis-lab stock is recorded. Their sequence files are still available. Whether a clone was in fact deposited and simply never recorded here is worth checking against DNASU during the revision.

Scope of this page. These are inconsistencies internal to the source data, found by comparing the legacy site against itself. No record has been checked against the current content of UniProt, NCBI or CAZy — that comparison is the substance of the record update and has not been attempted here.