OGA
Protein O-GlcNAcase and inactive Histone acetyltransferase
Glycoside hydrolaseGH84Clones available from DNASUAlso known as MGEA5
External resources
UniProt O60502AlphaFold modelInterPro domainsNCBI Gene 10724RefSeq NM_012215Protein NP_036347CAZy GH84DNASU clone search
Identification and annotation
| Gene symbol | OGA |
|---|---|
| Synonyms recorded in the Repository |
|
| Enzyme class | Glycoside hydrolase |
| CAZy family | GH84 (CAZy entry) |
| UniProt accession | O60502 |
| NCBI GeneID | 10724 |
| DNA RefSeq | NM_012215 |
| Protein RefSeq | NP_036347 |
| MGC accession (PCR template) | BC039583 and gene synthesis |
| Annotation | Protein O-GlcNAcase and inactive Histone acetyltransferase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | Cytosolic |
|---|---|
| Truncation strategy (eukaryotic hosts) | Full length/N-term fusion (from MCG clone), Full length/C-term fusion (gene synthesis) |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | GenBank (unavailable) | HsCD00358408 |
| C-term pDONR221 capture clone | C-term fusion | not yet assigned |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pGEn1 | N-term fusion | GenBank (unavailable) | HsCD00413371 |
| pGEn2 | N-term fusion | GenBank (unavailable) | HsCD00413186 |
| pGEn3 | N-term fusion | GenBank (unavailable) | HsCD00522512 |
| pGEc1 | C-term fusion | — | not applicable |
| pGEc2 | C-term fusion | not yet assigned |
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | — | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | GenBank (unavailable) | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.