POGLUT1
Protein O-glucosyltransferase 1
GlycosyltransferaseBaculovirus stock from the Jarvis labAlso known as KTELC1, RUMISource values disagree
Conflicting values in the source site. The original site stated this gene's identity in two places and they do not agree:
- UniProt accession: the GT90 family index recorded
Q8NBL1, this record recordedC9JYJ3. - NCBI GeneID: the GT90 family index recorded
56983, this record recordedGene Synthesis.
External resources
UniProt C9JYJ3AlphaFold modelInterPro domainsRefSeq NM_020231/NM_152305Protein 56983DNASU clone search
Identification and annotation
| Gene symbol | POGLUT1 |
|---|---|
| Synonyms recorded in the Repository |
|
| Enzyme class | Glycosyltransferase |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | C9JYJ3 |
| NCBI GeneID | Gene Synthesis |
| DNA RefSeq | NM_020231/NM_152305 |
| Protein RefSeq | 56983 |
| Annotation | Protein O-glucosyltransferase 1 |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | N-term SS + Cterm KTEL seq |
|---|---|
| Truncation strategy (eukaryotic hosts) | N-term del/N-term fusion: C-term deletion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | not yet assigned | |
| C-term pDONR221 capture clone | C-term fusion | — | not applicable |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pGEn1 | N-term fusion | — | not applicable |
| pGEn2 | N-term fusion | not yet assigned | |
| pGEn3 | N-term fusion | — | not applicable |
| pGEc1 | C-term fusion | — | not applicable |
| pGEc2 | C-term fusion | — | not applicable |
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | GenBank (unavailable) | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | GenBank (unavailable) | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.