ALG8
Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
GlycosyltransferaseGT57Clones available from DNASUDuplicated symbol
This symbol appears on more than one legacy record. The original site held two separate pages whose gene-symbol cell reads
ALG8. This page is built from ALG8.html, and the constructs below are the ones that page listed. Compare it with the other record of the same name before relying on either — see Data Notes.External resources
UniProt Q9BVK2AlphaFold modelInterPro domainsNCBI Gene 79053RefSeq NM_024079/NM_001007027Protein NP_076984/NM_001007027CAZy GT57DNASU clone search
Identification and annotation
| Gene symbol | ALG8 |
|---|---|
| Enzyme class | Glycosyltransferase |
| CAZy family | GT57 (CAZy entry) |
| UniProt accession | Q9BVK2 |
| NCBI GeneID | 79053 |
| DNA RefSeq | NM_024079/NM_001007027 |
| Protein RefSeq | NP_076984/NM_001007027 |
| MGC accession (PCR template) | BC001133 |
| Annotation | Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | Multipass |
|---|---|
| Truncation strategy (eukaryotic hosts) | Full length/C-term fusion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | — | not applicable |
| C-term pDONR221 capture clone | C-term fusion | HsCD00451097 |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | Request virus stock from the Jarvis lab | |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | — | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.