Glyco-Enzyme Repository

Repository of Expression Constructs for Glycosylation Enzymes

Gateway® entry clones and mammalian, baculovirus and bacterial expression constructs for glycosyltransferases, glycoside hydrolases and glycan-modifying enzymes

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ALG8

Dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

GlycosyltransferaseGT57Clones available from DNASUSource values disagreeDuplicated symbol
This symbol appears on more than one legacy record. The original site held two separate pages whose gene-symbol cell reads ALG8. This page is built from ALG6.html, and the constructs below are the ones that page listed. Compare it with the other record of the same name before relying on either — see Data Notes.
Conflicting values in the source site. The original site stated this gene's identity in two places and they do not agree:
  • Gene symbol: the GT57 family index recorded ALG6, this record recorded ALG8.
  • UniProt accession: the GT57 family index recorded Q9Y672, this record recorded Q9BVK2.
  • NCBI GeneID: the GT57 family index recorded 29929, this record recorded 79053.
Both readings are shown here because the migration does not decide which is right. The construct sequence files are the authority on what is actually in each plasmid. All such cases are listed on the Data Notes page.

External resources

UniProt Q9BVK2AlphaFold modelInterPro domainsNCBI Gene 79053RefSeq NM_024079/NM_001007027Protein NP_076984/NM_001007027CAZy GT57DNASU clone search

Identification and annotation

Gene symbolALG8
Enzyme classGlycosyltransferase
CAZy familyGT57 (CAZy entry)
UniProt accessionQ9BVK2
NCBI GeneID79053
DNA RefSeqNM_024079/NM_001007027
Protein RefSeqNP_076984/NM_001007027
MGC accession (PCR template)BC001133
AnnotationDolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase

Design strategy

How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.

Domain structure / topologyMultipass
Truncation strategy (eukaryotic hosts)Full length/C-term fusion

Gateway® entry clones

Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.

Entry cloneFusion strategySequence filesDNASU clone ID
N-term pDONR221 capture cloneN-term fusion—not applicable
C-term pDONR221 capture cloneC-term fusion
GenBank
HsCD00451131

Expression constructs

Mammalian Expression

Transient transfection of HEK293 cells. Clones are distributed by DNASU.

VectorFusion strategySequence filesDNASU clone ID
pGEn1N-term fusion—not applicable
pGEn2N-term fusion—not applicable
pGEn3N-term fusion—not applicable
pGEc1C-term fusion
GenBank
HsCD00413735
pGEc2C-term fusion
GenBank
HsCD00521208

Baculovirus Expression

Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.

VectorFusion strategySequence filesDNASU clone ID
Ac-polh-CtermHisStrep-DESTC-term fusion
GenBank
Request virus stock
from the Jarvis lab
Ac-polh-NtermMelHisStrep-DESTN-term fusion—Request virus stock
from the Jarvis lab
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.
Vector referenceMammalian vector designs (PDF)Baculovirus vector designs (PDF)
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