GCNT2C
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase, isoform C (I-branching enzyme)
GlycosyltransferaseGT14Baculovirus stock from the Jarvis labSource values disagree
Conflicting values in the source site. The original site stated this gene's identity in two places and they do not agree:
- UniProt accession: the GT14 family index recorded
Q8NFS9, this record recordedQ06430.
External resources
UniProt Q06430AlphaFold modelInterPro domainsNCBI Gene 2651RefSeq NM_001491Protein NP_001482CAZy GT14DNASU clone search
Identification and annotation
| Gene symbol | GCNT2C |
|---|---|
| Enzyme class | Glycosyltransferase |
| CAZy family | GT14 (CAZy entry) |
| UniProt accession | Q06430 |
| NCBI GeneID | 2651 |
| DNA RefSeq | NM_001491 |
| Protein RefSeq | NP_001482 |
| Annotation | N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase, isoform C (I-branching enzyme) |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | Type II TMD |
|---|---|
| Truncation strategy (eukaryotic hosts) | N-term deletion/N-term fusion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | not yet assigned | |
| C-term pDONR221 capture clone | C-term fusion | — | not applicable |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pGEn1 | N-term fusion | — | not applicable |
| pGEn2 | N-term fusion | not yet assigned | |
| pGEn3 | N-term fusion | — | not applicable |
| pGEc1 | C-term fusion | — | not applicable |
| pGEc2 | C-term fusion | — | not applicable |
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | — | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | GenBank (unavailable) | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.