CHST14
Carbohydrate sulfotransferase 14: synthesis of GalNAc-4-SO4 in dermatan sulfate
Glycan-modifying enzymeClones available from DNASUAlso known as D4ST1Duplicated symbol
This symbol appears on more than one legacy record. The original site held two separate pages whose gene-symbol cell reads
CHST14 (D4ST1). This page is built from CHST14.html, and the constructs below are the ones that page listed. Compare it with the other record of the same name before relying on either — see Data Notes.External resources
UniProt Q8NCH0AlphaFold modelInterPro domainsNCBI Gene 113189RefSeq NM_130468Protein NP_569735DNASU clone search
Identification and annotation
| Gene symbol | CHST14 |
|---|---|
| Synonyms recorded in the Repository |
|
| Enzyme class | Glycan-modifying enzyme |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q8NCH0 |
| NCBI GeneID | 113189 |
| DNA RefSeq | NM_130468 |
| Protein RefSeq | NP_569735 |
| MGC accession (PCR template) | BC053633 |
| Annotation | Carbohydrate sulfotransferase 14: synthesis of GalNAc-4-SO4 in dermatan sulfate |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | Type II-TMD |
|---|---|
| Truncation strategy (eukaryotic hosts) | N-term deletion/N-term fusion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | HsCD00522614 | |
| C-term pDONR221 capture clone | C-term fusion | — | not applicable |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | — | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.