GBE1
1,4-alpha-glucan-branching enzyme
Glycoside hydrolaseGH13Clones available from DNASU
External resources
UniProt Q04446AlphaFold modelInterPro domainsNCBI Gene 2632RefSeq NM_000158Protein NP_000149CAZy GH13DNASU clone search
Identification and annotation
| Gene symbol | GBE1 |
|---|---|
| Enzyme class | Glycoside hydrolase |
| CAZy family | GH13 (CAZy entry) |
| UniProt accession | Q04446 |
| NCBI GeneID | 2632 |
| DNA RefSeq | NM_000158 |
| Protein RefSeq | NP_000149 |
| MGC accession (PCR template) | BC012098 |
| Annotation | 1,4-alpha-glucan-branching enzyme |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | Cytosolic |
|---|---|
| Truncation strategy (eukaryotic hosts) | N-term deletion/N-term fusion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | HsCD00358380 | |
| C-term pDONR221 capture clone | C-term fusion | — | not applicable |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | — | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.