MANEAL
Glycoprotein endo-alpha-1,2-mannosidase-like protein
Glycoside hydrolaseGH99Baculovirus stock from the Jarvis lab
External resources
UniProt Q5VSG8AlphaFold modelInterPro domainsNCBI Gene 149175RefSeq NM_001031740, NM_152496Protein NP_001026910, NP_689709CAZy GH99DNASU clone search
Identification and annotation
| Gene symbol | MANEAL |
|---|---|
| Enzyme class | Glycoside hydrolase |
| CAZy family | GH99 (CAZy entry) |
| UniProt accession | Q5VSG8 |
| NCBI GeneID | 149175 |
| DNA RefSeq | NM_001031740, NM_152496 |
| Protein RefSeq | NP_001026910, NP_689709 |
| Annotation | Glycoprotein endo-alpha-1,2-mannosidase-like protein |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | Type II-TMD |
|---|---|
| Truncation strategy (eukaryotic hosts) | N-term deletion/N-term fusion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | not yet assigned | |
| C-term pDONR221 capture clone | C-term fusion | — | not applicable |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pGEn1 | N-term fusion | — | not applicable |
| pGEn2 | N-term fusion | — | not applicable |
| pGEn3 | N-term fusion | — | not applicable |
| pGEc1 | C-term fusion | — | not applicable |
| pGEc2 | C-term fusion | not yet assigned |
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | — | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | — | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.