SPAM1
Hyaluronidase PH-20
Glycoside hydrolaseGH56Clones available from DNASU
External resources
UniProt P38567AlphaFold modelInterPro domainsNCBI Gene 6677RefSeq NM_153189Protein NP_694859CAZy GH56DNASU clone search
Identification and annotation
| Gene symbol | SPAM1 |
|---|---|
| Enzyme class | Glycoside hydrolase |
| CAZy family | GH56 (CAZy entry) |
| UniProt accession | P38567 |
| NCBI GeneID | 6677 |
| DNA RefSeq | NM_153189 |
| Protein RefSeq | NP_694859 |
| MGC accession (PCR template) | BC026163 |
| Annotation | Hyaluronidase PH-20 |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | N-term sig seq |
|---|---|
| Truncation strategy (eukaryotic hosts) | N-term del/N-term fusion, Full length/C-term fusion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | HsCD00358362 | |
| C-term pDONR221 capture clone | C-term fusion | HsCD00451113 |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | Request virus stock from the Jarvis lab | |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.