CPF_0285
endo-N-acetylglucosaminidase · Clostridium perfringens ATCC 13124
Bacterial GT / GHClones available from DNASU
External resources
UniProt Q0TUE5AlphaFold modelInterPro domainsNCBI Gene 4201313RefSeq NC_008261.1Protein YP_694745DNASU clone search
Identification and annotation
| Gene symbol | CPF_0285 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q0TUE5 |
| NCBI GeneID | 4201313 |
| DNA RefSeq | NC_008261.1 |
| Protein RefSeq | YP_694745 |
| Source species | Clostridium perfringens ATCC 13124 |
| Annotation | endo-N-acetylglucosaminidase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 25-645 |
|---|---|
| Coding-region positions amplified | 103-2124 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | CpCD00405629 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | CpCD00405571 | |
| pET32-DEST | N-term fusion | CpCD00405513 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.