ClocelDRAFT_2582
glycosyltransferase family 2 or family 27 · Clostridium cellulovorans 743B
Bacterial GT / GHClones available from DNASU
External resources
UniProt C5RKN9AlphaFold modelInterPro domainsRefSeq NZ_ACPD01000026Protein ZP_04806013DNASU clone search
Identification and annotation
| Gene symbol | ClocelDRAFT_2582 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | C5RKN9 |
| NCBI GeneID | not recorded |
| DNA RefSeq | NZ_ACPD01000026 |
| Protein RefSeq | ZP_04806013 |
| Source species | Clostridium cellulovorans 743B |
| Annotation | glycosyltransferase family 2 or family 27 |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 2-297 |
|---|---|
| Coding-region positions amplified | 4-891 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | CcCD00405613 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | CcCD00405555 | |
| pET32-DEST | N-term fusion | CcCD00405497 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.