Glyco-Enzyme Repository

Repository of Expression Constructs for Glycosylation Enzymes

Gateway® entry clones and mammalian, baculovirus and bacterial expression constructs for glycosyltransferases, glycoside hydrolases and glycan-modifying enzymes

PMN2A_1233

glycosyltransferase family 41; entire gene, N-ter TPR repeats of BGT41_T01a1 · Prochlorococcus marinus str. NATL2A

Bacterial GT / GHClones available from DNASU

External resources

Identification and annotation

Gene symbolPMN2A_1233
Enzyme classBacterial GT / GH
CAZy familyNot assigned to a CAZy family
UniProt accessionQ46IF5
NCBI GeneID3606626
DNA RefSeqNC_007335.2
Protein RefSeqYP_292426
Source speciesProchlorococcus marinus str. NATL2A
Annotationglycosyltransferase family 41; entire gene, N-ter TPR repeats of BGT41_T01a1

Design strategy

How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.

Expressed residues2-509
Coding-region positions amplified4-1527

Expression constructs

Bacterial Expression

pET-based expression in E. coli. Clones are distributed by DNASU.

VectorFusion strategySequence filesDNASU clone ID
pET16-DESTN-term fusionPmCD00405550
pET32-DESTN-term fusionPmCD00405492
pET16-DESTN-term fusionPmCD00405595
pET32-DESTN-term fusionPmCD00405537
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.