fucT
Alpha-1,3-fucosyl-transferase; entire gene · Helicobacter pylori J99
Bacterial GT / GHClones available from DNASU
External resources
UniProt Q9ZLI3AlphaFold modelInterPro domainsNCBI Gene 890321RefSeq NC_000921Protein NP_223314DNASU clone search
Identification and annotation
| Gene symbol | fucT |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q9ZLI3 |
| NCBI GeneID | 890321 |
| DNA RefSeq | NC_000921 |
| Protein RefSeq | NP_223314 |
| Source species | Helicobacter pylori J99 |
| Annotation | Alpha-1,3-fucosyl-transferase; entire gene |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 2-454 |
|---|---|
| Coding-region positions amplified | 4-1362 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | HpCD00405597 | |
| pET32-DEST | N-term fusion | HpCD00405539 | |
| pET16-DEST | N-term fusion | HpCD00405567 | |
| pET32-DEST | N-term fusion | HpCD00405509 | |
| pET16-DEST | N-term fusion | HpCD00405542 | |
| pET32-DEST | N-term fusion | HpCD00405484 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.