mll0582
lipopolysaccharide biosynthesis protein · Mesorhizobium loti MAFF303099
Bacterial GT / GHClones available from DNASU
External resources
UniProt Q98MH1AlphaFold modelInterPro domainsNCBI Gene 1225019RefSeq NC_002678.2Protein NP_102356DNASU clone search
Identification and annotation
| Gene symbol | mll0582 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q98MH1 |
| NCBI GeneID | 1225019 |
| DNA RefSeq | NC_002678.2 |
| Protein RefSeq | NP_102356 |
| Source species | Mesorhizobium loti MAFF303099 |
| Annotation | lipopolysaccharide biosynthesis protein |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 2-931 |
|---|---|
| Coding-region positions amplified | 4-2793 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | MlCD00405594 | |
| pET32-DEST | N-term fusion | MlCD00405536 | |
| pET16-DEST | N-term fusion | MlCD00405590 | |
| pET32-DEST | N-term fusion | MlCD00405532 | |
| pET16-DEST | N-term fusion | MlCD00405598 | |
| pET32-DEST | N-term fusion | MlCD00405540 | |
| pET16-DEST | N-term fusion | MlCD00405549 | |
| pET32-DEST | N-term fusion | MlCD00405491 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.