wlaF
oligosaccharyltransferase [E.C. 2.4.1.119] · Campylobacter jejuni subsp. jejuni 81-176
Bacterial GT / GHClones available from DNASU
External resources
Identification and annotation
| Gene symbol | wlaF |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | A1W0B2 |
| NCBI GeneID | not recorded |
| DNA RefSeq | AF387298.1 |
| Protein RefSeq | AAK97438 |
| Source species | Campylobacter jejuni subsp. jejuni 81-176 |
| Annotation | oligosaccharyltransferase [E.C. 2.4.1.119] |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 426-713 |
|---|---|
| Coding-region positions amplified | 1279-2139 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | CjCD00405609 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | CjCD00405551 | |
| pET32-DEST | N-term fusion | CjCD00405493 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.