ISPD
Isoprenoid synthase domain-containing protein, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein
Glycan-modifying enzymeBaculovirus stock from the Jarvis lab
External resources
UniProt A4D126AlphaFold modelInterPro domainsNCBI Gene 729920RefSeq NM_001101426Protein NP_001094896DNASU clone search
Identification and annotation
| Gene symbol | ISPD |
|---|---|
| Enzyme class | Glycan-modifying enzyme |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | A4D126 |
| NCBI GeneID | 729920 |
| DNA RefSeq | NM_001101426 |
| Protein RefSeq | NP_001094896 |
| Annotation | Isoprenoid synthase domain-containing protein, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like protein |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | cytosolic |
|---|---|
| Truncation strategy (eukaryotic hosts) | N-term fusion with His tag |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pDONR221 capture clone | N-term fusion | not yet assigned |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pGEn1 | N-term fusion | — | not applicable |
| pGEn2 | N-term fusion | — | not applicable |
| pGEn3 | N-term fusion | — | not applicable |
| pGEc1 | N-term His fusion | not yet assigned | |
| pGEc2 | C-term fusion | — | not applicable |
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | — | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | — | Request virus stock from the Jarvis lab |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.