BT_0771
1,4-alpha-glucan branching enzyme (Isoamylase or pullulanase type II) + Cter · Bacteroides thetaiotaomicron VPI-5482
Bacterial GT / GHClones available from DNASU
External resources
UniProt Q8A9P4AlphaFold modelInterPro domainsNCBI Gene 1073748RefSeq NC_004663Protein NP_809684DNASU clone search
Identification and annotation
| Gene symbol | BT_0771 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q8A9P4 |
| NCBI GeneID | 1073748 |
| DNA RefSeq | NC_004663 |
| Protein RefSeq | NP_809684 |
| Source species | Bacteroides thetaiotaomicron VPI-5482 |
| Annotation | 1,4-alpha-glucan branching enzyme (Isoamylase or pullulanase type II) + Cter |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 136-670 |
|---|---|
| Coding-region positions amplified | 406-2060 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | BtCD00405554 | |
| pET32-DEST | N-term fusion | BtCD00405496 | |
| pET16-DEST | N-term fusion | BtCD00405562 | |
| pET32-DEST | N-term fusion | BtCD00405504 | |
| pET16-DEST | N-term fusion | BtCD00405586 | |
| pET32-DEST | N-term fusion | BtCD00405528 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.