BT_0458
beta-mannosidase · Bacteroides thetaiotaomicron VPI-5482
Bacterial GT / GHClones available from DNASU
External resources
UniProt Q8AAK6AlphaFold modelInterPro domainsNCBI Gene 1071631RefSeq NC_004663Protein NP_809371.1DNASU clone search
Identification and annotation
| Gene symbol | BT_0458 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q8AAK6 |
| NCBI GeneID | 1071631 |
| DNA RefSeq | NC_004663 |
| Protein RefSeq | NP_809371.1 |
| Source species | Bacteroides thetaiotaomicron VPI-5482 |
| Annotation | beta-mannosidase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 26-864 |
|---|---|
| Coding-region positions amplified | 76-2592 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | BtCD00405650 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | BtCD00405592 | |
| pET32-DEST | N-term fusion | BtCD00405534 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.