Cpin_3125
alpha-N-acetylglucosaminidase · Chitinophaga pinensis DSM 2588
Bacterial GT / GHClones available from DNASU
External resources
UniProt C7PPF7AlphaFold modelInterPro domainsNCBI Gene 8359290RefSeq NC_013132.1Protein YP_003122794DNASU clone search
Identification and annotation
| Gene symbol | Cpin_3125 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | C7PPF7 |
| NCBI GeneID | 8359290 |
| DNA RefSeq | NC_013132.1 |
| Protein RefSeq | YP_003122794 |
| Source species | Chitinophaga pinensis DSM 2588 |
| Annotation | alpha-N-acetylglucosaminidase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 22-728 |
|---|---|
| Coding-region positions amplified | 64-2184 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | CpCD00405633 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | CpCD00405575 | |
| pET32-DEST | N-term fusion | CpCD00405517 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.