Cthe_0212
Beta-glucosidase · Clostridium thermocellum ATCC 27405, anaerobic/thermophilic
Bacterial GT / GHClones available from DNASU
External resources
UniProt P26208AlphaFold modelInterPro domainsNCBI Gene 4808630RefSeq NC_009012.1Protein YP_001036646DNASU clone search
Identification and annotation
| Gene symbol | Cthe_0212 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | P26208 |
| NCBI GeneID | 4808630 |
| DNA RefSeq | NC_009012.1 |
| Protein RefSeq | YP_001036646 |
| Source species | Clostridium thermocellum ATCC 27405, anaerobic/thermophilic |
| Annotation | Beta-glucosidase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 2-471 |
|---|---|
| Coding-region positions amplified | 4-1413 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | CtCD00405602 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | CtCD00405544 | |
| pET32-DEST | N-term fusion | CtCD00405486 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.