STER_0149
dolichol-phosphate-β-D-mannosyltransferase · Streptococcus thermophilus LMD-9
Bacterial GT / GHClones available from DNASU
External resources
UniProt Q03MS9AlphaFold modelInterPro domainsNCBI Gene 4438301RefSeq NC_008532.1Protein YP_819689DNASU clone search
Identification and annotation
| Gene symbol | STER_0149 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q03MS9 |
| NCBI GeneID | 4438301 |
| DNA RefSeq | NC_008532.1 |
| Protein RefSeq | YP_819689 |
| Source species | Streptococcus thermophilus LMD-9 |
| Annotation | dolichol-phosphate-β-D-mannosyltransferase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 2-397 |
|---|---|
| Coding-region positions amplified | 4-1191 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | StCD00405630 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | StCD00405572 | |
| pET32-DEST | N-term fusion | StCD00405514 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.