TM0306
alpha-L-fucosidase · Thermotoga maritima MSB8
Bacterial GT / GHClones available from DNASU
External resources
UniProt Q9WYE2AlphaFold modelInterPro domainsNCBI Gene 897238RefSeq NC_000853Protein AAD35394.1DNASU clone search
Identification and annotation
| Gene symbol | TM0306 |
|---|---|
| Enzyme class | Bacterial GT / GH |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | Q9WYE2 |
| NCBI GeneID | 897238 |
| DNA RefSeq | NC_000853 |
| Protein RefSeq | AAD35394.1 |
| Source species | Thermotoga maritima MSB8 |
| Annotation | alpha-L-fucosidase |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Expressed residues | 2-449 |
|---|---|
| Coding-region positions amplified | 4-1347 |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | TmCD00405642 |
Expression constructs
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| pET16-DEST | N-term fusion | TmCD0005584 | |
| pET32-DEST | N-term fusion | TmCD00405526 |
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.