CHST1
Homo sapiens carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1), mRNA
Glycan-modifying enzymeClones available from DNASU
External resources
Identification and annotation
| Gene symbol | CHST1 |
|---|---|
| Enzyme class | Glycan-modifying enzyme |
| CAZy family | Not assigned to a CAZy family |
| UniProt accession | O43916 |
| NCBI GeneID | 8534 |
| DNA RefSeq | NM_003654 |
| Protein RefSeq | not recorded |
| MGC accession (PCR template) | BC022567 |
| Annotation | Homo sapiens carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1), mRNA |
Design strategy
How this coding region was truncated for soluble expression. See Construct Design for the strategy behind these choices.
| Domain structure / topology | N-term sig seq |
|---|---|
| Truncation strategy (eukaryotic hosts) | None |
| Truncation strategy (bacterial host) | N-term del/N-term fusion |
Gateway® entry clones
Coding regions captured in pDONR221 (or an equivalent synthesised donor). These transfer into any Gateway destination vector by LR recombination, including user-supplied vectors for custom projects.
| Entry clone | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| N-term pDONR221 capture clone | N-term fusion | HsCD00522604 | |
| C-term pDONR221 capture clone | C-term fusion | — | not applicable |
Expression constructs
Mammalian Expression
Transient transfection of HEK293 cells. Clones are distributed by DNASU.
Baculovirus Expression
Recombinant baculovirus for expression in Sf9 or other lepidopteran insect cells. Virus stocks are supplied by the Jarvis lab, not DNASU.
| Vector | Fusion strategy | Sequence files | DNASU clone ID |
|---|---|---|---|
| Ac-polh-CtermHisStrep-DEST | C-term fusion | — | Request virus stock from the Jarvis lab |
| Ac-polh-NtermMelHisStrep-DEST | N-term fusion | Request virus stock from the Jarvis lab |
Bacterial Expression
pET-based expression in E. coli. Clones are distributed by DNASU.
Ordering. Copy the DNASU clone ID and paste it into the DNASU clone search. Rows marked not yet assigned describe a construct design for which no distributable clone has been deposited. Baculovirus stocks are supplied by the Jarvis lab (dljarvis@uwyo.edu), never by DNASU. A step-by-step walkthrough is on the Tutorial page.