Glycoanalytics services
The BioF:GREAT User Facility, operating within the CCRC Analytical Services and Training Laboratory, provides carbohydrate and glycoconjugate analysis to academic, government and industrial users. Services run from monosaccharide composition through site-specific glycoproteomics, using HPAEC, GC-MS, MALDI-TOF/TOF, Orbitrap MS and NMR.
Available services
N- and O-linked glycomics
Profiling of released N- and O-linked glycans from purified glycoproteins, cells, tissues, culture supernatants and gel bands by MALDI-TOF/TOF and Orbitrap mass spectrometry.
Glycoproteomics
Site-specific characterisation of protein glycosylation by protease digestion and ETD/HCD tandem mass spectrometry.
Glycolipids and additional analyses
Extraction and characterisation of glycosphingolipids and glycosylphosphatidylinositols, plus sulfation/phosphorylation analysis, SDS-PAGE, in-gel digestion and lectin blotting.
Glycan sequencing by tandem MS
Sequence determination of released glycans by tandem mass spectrometry.
Monosaccharide and linkage analysis
Monosaccharide composition by HPAEC and GC-MS, glycosyl linkage analysis by methylation GC-MS, and exoglycosidase digestion.
Sialic acid analysis
Composition, linkage and side-chain modification analysis of sialic acids, including O-acetylation.
Sites of glycosylation
N- and O-linked site mapping, including 18O-labelling and LC-MS isotopic shift monitoring.
How to request a service
- Identify the analysis. Select the service area above; each page lists the methods used, the information returned and typical sample requirements.
- Submit a request. Complete the service request form describing the sample, its origin and the question you need answered.
- Consultation. Facility staff confirm the analytical approach, sample amount, turnaround time and cost.
- Sample submission. Ship the sample with the agreed documentation.
- Report. You receive processed data with an interpretation, and follow-up consultation where the result warrants it.